The tour supported his second studio album, Unorthodox Jukebox (2012). After an official announcement on February 10, 2013, which coincided with Mars' performance at the 55th Annual Grammy Awards, a promotional trailer and behind-the-scenes…
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A clone selected for sequencing (VP-cosC6) was subcloned and the sequencing templates from individual subclones were prepared using the GeneJumper primer insertion kit (Invitrogen, San Diego).